Design and fabrication of hybrid triple-responsive κ-carrageenan-based nanospheres for controlled drug delivery

Abstract

In the last two decades, the utilization of magnetic nanospheres in intelligent polymeric structures have received increased attention of researchers in numerous biomedical applications. Here, hybrid nanostructured triple-responsive magnetic nanospheres (κ-Car-g-P(AA/DMA)@Fe3O4) containing inorganic iron oxide core (Fe3O4) and organic graft copolymeric shell based on κ-carrageenan (κ-Car) and poly(acrylic acid/dimethylaminoethyl methacrylate) (P(AA/DMA)) were synthesized by microwave induced co-precipitation technique. The structure, size, surface morphology, magnetic property and stability of synthesized κ-Car-g-P(AA/DMA)@Fe3O4 magnetic nanospheres were characterized using FTIR, UV, XRD, TEM, Zeta-sizer, and VSM. κ-Car-g-P(AA/DMA)@Fe3O4 nanospheres were loaded with 5-Fluorouracil (5-FU) as an antineoplastic drug, and their 5-FU release behavior was explored in diverse graft yields, pH values, temperatures and in the existence of an alternating magnetic field. The κ-Car-g-P(AA/DMA)@Fe3O4 nanospheres demonstrated pH-, thermo-, and magnetic field-responsive 5-FU release with good biocompatibility and excellent anticancer activity. In addition, 5-FU release under 50 mT magnetic field reached to 100% within 4 h. This work exhibits that hybrid nanospheres have a triple stimuli-responsive influence, which is of principal importance for the future design and application of multi-functional responsive platforms to develop externally stimulated release of active agents and their healthcare capability.